Rotavirus VP6 Proteins expressed in Lactobacillus plantarum NC8. CURRENT STATUS: POSTED. SERIA SHONYELA. Sokoine University of Agriculture.

Apr 14, 2020 Lactobacillus plantarum is one of the important lactobacilli that has bacteriocins PlnJK, PlnEF and plantaricin NC8 possess antimicrobial

Plantaricin NC8, a two-peptide non-lantibiotic class IIb bacteriocin composed of PLNC8α and PLNC8β and derived from Lactobacillus plantarum ZJ316, has been shown to be highly potent against a range of bacteria and fungi. Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many laboratories worldwide. Plantaricin NC8, a two-peptide non-lantibiotic class IIb bacteriocin composed of PLNC8α and PLNC8β and derived from Lactobacillus plantarum ZJ316, has been shown to be highly potent against a range of bacteria and fungi. A new, coculture-inducible two-peptide bacteriocin named plantaricin NC8 (PLNC8) was isolated from Lactobacillus plantarum NC8 cultures which had been induced with Lactococcus lactis MG1363 or Pediococcus pentosaceus FBB63. Recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 in mice 161 www.vetsci.org Fig. 1.

plantarum NC8 genome sequencing project Read "Lactonase-expressing Lactobacillus plantarum NC8 attenuates the virulence factors of multiple drug resistant Pseudomonas aeruginosa in co-culturing environment, World Journal of Microbiology and Biotechnology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. ABSTRACT Blanched and nonblanched potato rods (var. Beate) were fermented with Lactobacillus plantarum strain NC8 (109 colony-forming units [CFU]/mL) … Lactobacillus plantarum NC8 cultures which had been induced with Lactococcus lactis MG1363 or Pediococcus pentosaceus FBB63. This bacteriocin consists of two distinct peptides, named and, which were separated by C 2-C 18 reverse-phase chromatography and whose complementary action is necessary for full plantaricin NC8 Transformation of Lb. sakei Lb790, Lb. plantarum NC8 and Lb. casei CECT475 with plasmids pSIP409UAI, pSIP411UAI and pMGUAI yielded recombinant Lactobacillus spp.-derived strains which were further checked by bacteriocinogenicity tests, PCR and sequencing of the inserts. particular, Lactobacillus plantarum strains can target a series of pathogens, such as Helicobacter felis (10), Mycobacterium tuberculosis (11), and even tumor antigens (12). In addition, recombinant L. plantarum NC8 strains expressing the M2e or HA2 antigen constructed by Yang et al.

We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many laboratories worldwide.

Plantaricin NC8, a two-peptide non-lantibiotic class IIb bacteriocin composed of PLNC8α and PLNC8β and derived from Lactobacillus plantarum ZJ316 Plantaricin NC8 from Lactobacillus plantarum causes cell membrane disruption to Micrococcus luteus without targeting lipid II | SpringerLink

In this study we examine the expression of heterologous oxalate decarboxylase (oxdc) in Lactobacillus plantarum NC8. Generally, this enzyme is not present in Lactobacillus spp. Oxdc gene from Bacillus Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract.

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particular, Lactobacillus plantarum strains can target a series of pathogens, such as Helicobacter felis (10), Mycobacterium tuberculosis (11), and even tumor antigens (12). In addition, recombinant L. plantarum NC8 strains expressing the M2e or HA2 antigen constructed by Yang et al. have a protective effect after H9N2 subtype avian inﬂuenza Lactobacillus plantarum NC8 cells harbouring pGM4‐gus were induced at an O.D. 600 of ca 0·3 by addition of pheromone (IP‐673) to a final concentration of 50 ng ml −1.

Lactobacillus plantarum NC8 strain, originally isolated from grass silage (Aukrust & Blom, 1992), was a generous gift from Dr Emmanuelle Maguin (Unité de Génétique Microbienne, I.N.R.A., Centre de Jouy-en-Josas, France). It was propagated using MRS broth (Difco) at 30 °C without shaking.
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Plantaricin NC8 (PLNC8), a coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8, has recently been purified and genetically characterized. Analysis of an 8.1-kb NC8 DNA region downstream of the PLNC8 operon revealed the presence of at least four operons involved in bacteriocin production, showing high homology to the plantaricin cluster in L. plantarum C11.

The molecular weight of Plantaricin NC8, a two-peptide non-lantibiotic class IIb bacteriocin composed of PLNC8α and PLNC8β and derived from Lactobacillus plantarum ZJ316, has been shown to be highly potent against a range of bacteria and fungi. 2020-12-01 · To develop a more effective and safe oral vaccine against rabies, we constructed recombinant Lactobacillus plantarum NC8 carrying one or two copies of the G gene with a dendritic cell-targeting peptide (DCpep) fused at the C-terminal designated NC8-pSIP409-sRVG or NC8-pSIP409-dRVG, respectively. A high expression system in Escherichia coli BL21 (DE3) was constructed to produce and characterise the biochemical properties of the MurI from Lactobacillus plantarum NC8. In a 4-L-bioreactor cultivation, 3266 nkat(D-Glu)/mg(protein) of specific enzyme activity was produced.

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Dendritic Cells Targeting Lactobacillus plantarum Strain NC8 with a Surface-Displayed Single-Chain Variable Fragment of CD11c Induce an Antigen-Specific Protective Cellular Immune Response Jing Liu , Guilian Yang , Haibin Huang , Chunwei Shi , Xing Gao , Wentao Yang , Zan Zhang , Yang Liu , Ke Xu , Jianzhong Wang , Yuanhuan Kang , Yanlong Jiang , Chunfeng Wang

Shonyela, S. , Yang, W. , Yang, G. and Wang, C. (2020) Innate Cytokine Responses and Toll-Like Receptor Induced by Recombinant Porcine Rotavirus VP6 and VP7 Proteins Expressing in Lactobacillus plantarum NC8 Strain Colonization in Mice. World Journal of Vaccines, 10, 17-31. doi: 10.4236/wjv.2020.101002. Production of plantaricin Nc8 by Lactobacillus plantarum Nc8 is induced in the presence of different types of gram-positive bacteria. Arch. Microbiol. 181, 8–16.